|
KMID : 0391119930020020058
|
|
Ulsan University Medical Journal
1993 Volume.2 No. 2 p.58 ~ p.63
|
|
|
The Detection of Enteroviruses using Enzymatic RNA amplification and Non-radioactive Labeled Nucleic Acid Hybridization Techniques
|
|
|
|
|
Abstract
|
|
|
The human enteroviruses (EVs) are responsible for various infections ranging in severity form asymptomatic to fatal. Since the clinical diagnosis of EV infection is complicated by the nonspecific nature of the clinical manifestations, Accurate
and
rapid
microbiological diagnosis of EV infection is very important for the management of patients.
This report describes polymerase chain reaction (PCR) and non-radioactive labeled nucleic acid hybridization techniques by which the enteroviral RNA can be amplified to a detectable level. The primer pair, derived from the highly conserved part
of
the
5'-noncoding region of the enteroviral genome, was selected in an effort to detect many different serotypes of EVs. After optimal conditions for PCR was determined, coxsackievirus B type 3 was detected at the level of 1 x 10E3 50% tissue culture
infective doses (TCID50). Our results demonstrate the applicability of PCR technique for the detection of enteroviral RNA from the clinical specimens.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|